Transcriptome of rat subcortical white matter and spinal cord after spinal injury and cortical stimulation

Bethany R. Kondiles; Haichao Wei; Lesley S. Chaboub; Philip J. Horner; Jia Qian Wu; Steve I. Perlmutter

doi:10.1038/s41597-021-00953-4

Transcriptome of rat subcortical white matter and spinal cord after spinal injury and cortical stimulation

Bethany R. Kondiles, Haichao Wei, Lesley S. Chaboub, Philip J. Horner, Jia Qian Wu, Steve I. Perlmutter

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Spinal cord injury disrupts ascending and descending neural signals causing sensory and motor dysfunction. Neuromodulation with electrical stimulation is used in both clinical and research settings to induce neural plasticity and improve functional recovery following spinal trauma. However, the mechanisms by which electrical stimulation affects recovery remain unclear. In this study we examined the effects of cortical electrical stimulation following injury on transcription at several levels of the central nervous system. We performed a unilateral, incomplete cervical spinal contusion injury in rats and delivered stimulation for one week to the contralesional motor cortex to activate the corticospinal tract and other pathways. RNA was purified from bilateral subcortical white matter and 3 levels of the spinal cord. Here we provide the complete data set in the hope that it will be useful for researchers studying electrical stimulation as a therapy to improve recovery from the deficits associated with spinal cord injury.

Original language	English (US)
Article number	175
Journal	Scientific Data
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41597-021-00953-4
State	Published - Dec 2021

ASJC Scopus subject areas

Statistics and Probability
Information Systems
Education
Computer Science Applications
Statistics, Probability and Uncertainty
Library and Information Sciences

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title = "Transcriptome of rat subcortical white matter and spinal cord after spinal injury and cortical stimulation",

abstract = "Spinal cord injury disrupts ascending and descending neural signals causing sensory and motor dysfunction. Neuromodulation with electrical stimulation is used in both clinical and research settings to induce neural plasticity and improve functional recovery following spinal trauma. However, the mechanisms by which electrical stimulation affects recovery remain unclear. In this study we examined the effects of cortical electrical stimulation following injury on transcription at several levels of the central nervous system. We performed a unilateral, incomplete cervical spinal contusion injury in rats and delivered stimulation for one week to the contralesional motor cortex to activate the corticospinal tract and other pathways. RNA was purified from bilateral subcortical white matter and 3 levels of the spinal cord. Here we provide the complete data set in the hope that it will be useful for researchers studying electrical stimulation as a therapy to improve recovery from the deficits associated with spinal cord injury.",

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note = "Funding Information: The work was supported by NIH grant R01NS099872. B.R.K. was funded in part by the NSF GRFP DGE-1256082 and DGE-1762114. Any opinion, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. H.W. and J.Q.W. were supported by the National Institutes of Health R01 NS088353 and R21EY028647-01; The Staman Ogilvie Fund-Memorial Hermann Foundation; and Mission Connect, a program of The Institute for Rehabilitation and Research (TIRR) Foundation. We acknowledge the Epigenomics Core of Weill Cornell Medical College for sequencing these samples. The authors acknowledge T.H. Lee, R. Robinson, and C. Ganji for assistance in conducting these experiments. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

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Transcriptome of rat subcortical white matter and spinal cord after spinal injury and cortical stimulation

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