Unique biomechanical interactions between myeloma cells and bone marrow stroma cells

Yongdong Feng; Gidon Ofek; Dong Soon Choi; Jianguo Wen; Jerry Hu; Hong Zhao; Youli Zu; Kyriacos A. Athanasiou; Chung Che Chang

doi:10.1016/j.pbiomolbio.2009.10.004

Unique biomechanical interactions between myeloma cells and bone marrow stroma cells

Yongdong Feng, Gidon Ofek, Dong Soon Choi, Jianguo Wen, Jerry Hu, Hong Zhao, Youli Zu, Kyriacos A. Athanasiou, Chung Che Chang

Research output: Contribution to journal › Review article › peer-review

12 Scopus citations

Abstract

We observed that BMSCs (bone marrow stromal cells) from myeloma patients (myeloma BMSCs) were significantly stiffer than control BMSCs using a cytocompression device. The stiffness of myeloma BMSCs and control BMSCs was further increased upon priming by myeloma cells. Additionally, myeloma cells became stiffer when primed by myeloma BMSCs. The focal adhesion kinase activity of myeloma cells was increased when cells were on stiffer collagen gels and on myeloma BMSCs. This change in myeloma stiffness is associated with increased colony formation of myeloma cells and FAK activation when co-cultured with stiffer myeloma BMSCs or stiffer collagen. Additionally, stem cells of RPMI8226 cells became stiffer after priming by myeloma BMSCs, with concomitant increases of stem cell colony formation. These results suggest the presence of a mechanotransduction loop between myeloma cells and myeloma BMSCs to increase the stiffness of both types of cells via FAK activation. The increase of stiffness may in turn support the growth of myeloma cells and myeloma stem cells.

Original language	English (US)
Pages (from-to)	148-156
Number of pages	9
Journal	Progress in Biophysics and Molecular Biology
Volume	103
Issue number	1
DOIs	https://doi.org/10.1016/j.pbiomolbio.2009.10.004
State	Published - Sep 2010

Keywords

Bone marrow stroma cell
Myeloma
Niche
Stiffness

ASJC Scopus subject areas

Molecular Biology
Biophysics

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10.1016/j.pbiomolbio.2009.10.004

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title = "Unique biomechanical interactions between myeloma cells and bone marrow stroma cells",

abstract = "We observed that BMSCs (bone marrow stromal cells) from myeloma patients (myeloma BMSCs) were significantly stiffer than control BMSCs using a cytocompression device. The stiffness of myeloma BMSCs and control BMSCs was further increased upon priming by myeloma cells. Additionally, myeloma cells became stiffer when primed by myeloma BMSCs. The focal adhesion kinase activity of myeloma cells was increased when cells were on stiffer collagen gels and on myeloma BMSCs. This change in myeloma stiffness is associated with increased colony formation of myeloma cells and FAK activation when co-cultured with stiffer myeloma BMSCs or stiffer collagen. Additionally, stem cells of RPMI8226 cells became stiffer after priming by myeloma BMSCs, with concomitant increases of stem cell colony formation. These results suggest the presence of a mechanotransduction loop between myeloma cells and myeloma BMSCs to increase the stiffness of both types of cells via FAK activation. The increase of stiffness may in turn support the growth of myeloma cells and myeloma stem cells.",

keywords = "Bone marrow stroma cell, Myeloma, Niche, Stiffness",

author = "Yongdong Feng and Gidon Ofek and Choi, {Dong Soon} and Jianguo Wen and Jerry Hu and Hong Zhao and Youli Zu and Athanasiou, {Kyriacos A.} and Chang, {Chung Che}",

note = "Funding Information: This study was supported by a The Methodist Hospital Research Institute (TMHRI) Scholarship grant and a Clinical and Translational Science Research Scholar grant by TMHRI.",

year = "2010",

month = sep,

doi = "10.1016/j.pbiomolbio.2009.10.004",

language = "English (US)",

volume = "103",

pages = "148--156",

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T1 - Unique biomechanical interactions between myeloma cells and bone marrow stroma cells

AU - Feng, Yongdong

AU - Ofek, Gidon

AU - Choi, Dong Soon

AU - Wen, Jianguo

AU - Hu, Jerry

AU - Zhao, Hong

AU - Zu, Youli

AU - Athanasiou, Kyriacos A.

AU - Chang, Chung Che

N1 - Funding Information: This study was supported by a The Methodist Hospital Research Institute (TMHRI) Scholarship grant and a Clinical and Translational Science Research Scholar grant by TMHRI.

PY - 2010/9

Y1 - 2010/9

N2 - We observed that BMSCs (bone marrow stromal cells) from myeloma patients (myeloma BMSCs) were significantly stiffer than control BMSCs using a cytocompression device. The stiffness of myeloma BMSCs and control BMSCs was further increased upon priming by myeloma cells. Additionally, myeloma cells became stiffer when primed by myeloma BMSCs. The focal adhesion kinase activity of myeloma cells was increased when cells were on stiffer collagen gels and on myeloma BMSCs. This change in myeloma stiffness is associated with increased colony formation of myeloma cells and FAK activation when co-cultured with stiffer myeloma BMSCs or stiffer collagen. Additionally, stem cells of RPMI8226 cells became stiffer after priming by myeloma BMSCs, with concomitant increases of stem cell colony formation. These results suggest the presence of a mechanotransduction loop between myeloma cells and myeloma BMSCs to increase the stiffness of both types of cells via FAK activation. The increase of stiffness may in turn support the growth of myeloma cells and myeloma stem cells.

AB - We observed that BMSCs (bone marrow stromal cells) from myeloma patients (myeloma BMSCs) were significantly stiffer than control BMSCs using a cytocompression device. The stiffness of myeloma BMSCs and control BMSCs was further increased upon priming by myeloma cells. Additionally, myeloma cells became stiffer when primed by myeloma BMSCs. The focal adhesion kinase activity of myeloma cells was increased when cells were on stiffer collagen gels and on myeloma BMSCs. This change in myeloma stiffness is associated with increased colony formation of myeloma cells and FAK activation when co-cultured with stiffer myeloma BMSCs or stiffer collagen. Additionally, stem cells of RPMI8226 cells became stiffer after priming by myeloma BMSCs, with concomitant increases of stem cell colony formation. These results suggest the presence of a mechanotransduction loop between myeloma cells and myeloma BMSCs to increase the stiffness of both types of cells via FAK activation. The increase of stiffness may in turn support the growth of myeloma cells and myeloma stem cells.

KW - Bone marrow stroma cell

KW - Myeloma

KW - Niche

KW - Stiffness

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ER -

Unique biomechanical interactions between myeloma cells and bone marrow stroma cells

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