TY - JOUR
T1 - Urine Dilution and Concentration After Digoxin Infusion into the Renal Artery of Dogs (34898)
AU - Martinez-Maldonado, Manuel
AU - Eknoyan, G.
AU - Allen, Julius C.
AU - Suki, Wadi N.
AU - Schwartz, A.
N1 - Funding Information:
Supported by Section Grant 101.122, Project 48–69, from the Veterans Administration, and grants from the USPHS (HE-05435-09, p. 8 and HE-07906-07), from the National Cystic Fibrosis Foundation, and from the National Science Foundation (GB 6895). 2
PY - 1970/7
Y1 - 1970/7
N2 - The present studies extend our observations of the effect of digoxin on renal concentrating capacity and, in addition, investigate its action on renal diluting capacity. Infusion of digoxin into a renal artery of dogs undergoing water diuresis resulted in increases in fractional urine flow (V/100 ml GFR) and fractional sodium clearance (CNa/100 ml GFR) to levels which could not be accounted for simply by distal inhibition, suggesting that the glycoside suppressed proximal reabsorption as well. Furthermore, CH2O was inhibited at any level of V/100 ml GFR examined. These effects of digoxin on TCH2O and CH2O indicate that it inhibits sodium transport in the ascending limb of Henle's loop. In view of our previous demonstration that the digoxin inhibition of TcH2O is accompanied by significant depression of Na+, K+-ATPase activity from medullary renal tissue, the present experiments are further evidence that the enzyme plays a fundamental role in the mechanism of urine concentration and dilution.
AB - The present studies extend our observations of the effect of digoxin on renal concentrating capacity and, in addition, investigate its action on renal diluting capacity. Infusion of digoxin into a renal artery of dogs undergoing water diuresis resulted in increases in fractional urine flow (V/100 ml GFR) and fractional sodium clearance (CNa/100 ml GFR) to levels which could not be accounted for simply by distal inhibition, suggesting that the glycoside suppressed proximal reabsorption as well. Furthermore, CH2O was inhibited at any level of V/100 ml GFR examined. These effects of digoxin on TCH2O and CH2O indicate that it inhibits sodium transport in the ascending limb of Henle's loop. In view of our previous demonstration that the digoxin inhibition of TcH2O is accompanied by significant depression of Na+, K+-ATPase activity from medullary renal tissue, the present experiments are further evidence that the enzyme plays a fundamental role in the mechanism of urine concentration and dilution.
UR - http://www.scopus.com/inward/record.url?scp=0014817387&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0014817387&partnerID=8YFLogxK
U2 - 10.3181/00379727-134-34898
DO - 10.3181/00379727-134-34898
M3 - Article
C2 - 5431377
AN - SCOPUS:0014817387
SN - 0037-9727
VL - 134
SP - 855
EP - 860
JO - Proceedings of the Society for Experimental Biology and Medicine
JF - Proceedings of the Society for Experimental Biology and Medicine
IS - 3
ER -