TY - JOUR
T1 - Whole-cell repetitive element sequence-based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates
AU - Woods, C. R.
AU - Versalovic, James
AU - Koeuth, T.
AU - Lupski, J. R.
PY - 1993
Y1 - 1993
N2 - Repetitive element sequence-based polymerase chain reaction (rep-PCR) enables the generation of DNA fingerprints which discriminate bacterial species and strains. We describe the application of whole-cell methods which allow specimens from broth cultures or colonies from agar plates to be utilized directly in the PCR mixture. The rep-PCR-generated DNA fingerprints obtained with whole-cell samples match results obtained with genomic DNA templates. Examples with different gram-negative bacteria (e.g., Citrobacter diversus, Escherichia coli, and Pseudomonas aeruginosa) and gram-positive bacteria (e.g., Staphylococcus aureus and Streptococcus pneumoniae) are demonstrated. Rapid specimen preparation methods enable rep-PCR-based fingerprinting to be competed in several hours and, therefore, allows the timely analysis of epidemiological relationships.
AB - Repetitive element sequence-based polymerase chain reaction (rep-PCR) enables the generation of DNA fingerprints which discriminate bacterial species and strains. We describe the application of whole-cell methods which allow specimens from broth cultures or colonies from agar plates to be utilized directly in the PCR mixture. The rep-PCR-generated DNA fingerprints obtained with whole-cell samples match results obtained with genomic DNA templates. Examples with different gram-negative bacteria (e.g., Citrobacter diversus, Escherichia coli, and Pseudomonas aeruginosa) and gram-positive bacteria (e.g., Staphylococcus aureus and Streptococcus pneumoniae) are demonstrated. Rapid specimen preparation methods enable rep-PCR-based fingerprinting to be competed in several hours and, therefore, allows the timely analysis of epidemiological relationships.
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U2 - 10.1128/jcm.31.7.1927-1931.1993
DO - 10.1128/jcm.31.7.1927-1931.1993
M3 - Comment/debate
C2 - 8349778
AN - SCOPUS:0027283317
SN - 0095-1137
VL - 31
SP - 1927
EP - 1931
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 7
ER -