TY - JOUR
T1 - Whole transcriptome analysis reveals a role for OGG1-initiated DNA repair signaling in airway remodeling
AU - Aguilera-Aguirre, Leopoldo
AU - Hosoki, Koa
AU - Bacsi, Attila
AU - Radák, Zsolt
AU - Sur, Sanjiv
AU - Hegde, Muralidhar L.
AU - Tian, Bing
AU - Saavedra-Molina, Alfredo
AU - Brasier, Allan R.
AU - Ba, Xueqing
AU - Boldogh, Istvan
N1 - Funding Information:
This work was supported by Grants NIEHS RO1 ES018948 (I.B.) and NIAID/AI062885 (A.R.B., I.B.), NHLBI Proteomic Center Grant N01HV00245 (I.B., S.S.; Dr. A. Kurosky, Director), NIEHS Center Grant P30 ES006676 , the International Science–Technology Collaboration Foundation ( 20120728 ) of Jilin Province in China (X.B.), the European Union and the European Social Fund , TAMOP 4.2.2.A-11/1/KONV-2012-2023 (A.B.). L. Aguilera-Aguirre is an Environmental Toxicology Research Training Fellow ( NIEHS T32 ES007254-22 ). We thank Mardelle Susman (Department of Microbiology and Immunology) for critically editing the manuscript and Dr. David Konkel (Institute for Translational Sciences, University of Texas Medical Branch) both for his scientific input and for editing the manuscript.
Publisher Copyright:
© 2015 Published by Elsevier Inc.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Reactive oxygen species (ROS) generated by environmental exposures, and endogenously as by-products of respiration, oxidatively modify biomolecules including DNA. Accumulation of ROS-induced DNA damage has been implicated in various diseases that involve inflammatory processes, and efficient DNA repair is considered critical in preventing such diseases. One of the most abundant DNA base lesions is 7,8-dihydro-8-oxoguanine (8-oxoG), which is repaired by the 8-oxoguanine DNA glycosylase 1 (OGG1)-initiated base-excision repair (OGG1-BER) pathway. Recent studies have shown that the OGG1-BER by-product 8-oxoG base forms a complex with cytosolic OGG1, activating small GTPases and downstream cell signaling in cultured cells and lungs. This implies that persistent OGG1-BER could result in signaling leading to histological changes in airways. To test this, we mimicked OGG1-BER by repeatedly challenging airways with its repair product 8-oxoG base. Gene expression was analyzed by RNA sequencing (RNA-Seq) and qRT-PCR, and datasets were evaluated by gene ontology and statistical tools. RNA-Seq analysis identified 3252 differentially expressed transcripts (2435 up- and 817 downregulated, ≥ 3-fold change). Among the upregulated transcripts, 2080 mRNAs were identified whose encoded protein products were involved in modulation of the actin family cytoskeleton, extracellular matrix, cell adhesion, cadherin, and cell junctions, affecting biological processes such as tissue development, cell-to-cell adhesion, cell communication, and the immune system. These data are supported by histological observations showing epithelial alterations, subepithelial fibrosis, and collagen deposits in the lungs. These data imply that continuous challenge by the environment and consequent OGG1-BER-driven signaling trigger gene expression consistent with airway remodeling.
AB - Reactive oxygen species (ROS) generated by environmental exposures, and endogenously as by-products of respiration, oxidatively modify biomolecules including DNA. Accumulation of ROS-induced DNA damage has been implicated in various diseases that involve inflammatory processes, and efficient DNA repair is considered critical in preventing such diseases. One of the most abundant DNA base lesions is 7,8-dihydro-8-oxoguanine (8-oxoG), which is repaired by the 8-oxoguanine DNA glycosylase 1 (OGG1)-initiated base-excision repair (OGG1-BER) pathway. Recent studies have shown that the OGG1-BER by-product 8-oxoG base forms a complex with cytosolic OGG1, activating small GTPases and downstream cell signaling in cultured cells and lungs. This implies that persistent OGG1-BER could result in signaling leading to histological changes in airways. To test this, we mimicked OGG1-BER by repeatedly challenging airways with its repair product 8-oxoG base. Gene expression was analyzed by RNA sequencing (RNA-Seq) and qRT-PCR, and datasets were evaluated by gene ontology and statistical tools. RNA-Seq analysis identified 3252 differentially expressed transcripts (2435 up- and 817 downregulated, ≥ 3-fold change). Among the upregulated transcripts, 2080 mRNAs were identified whose encoded protein products were involved in modulation of the actin family cytoskeleton, extracellular matrix, cell adhesion, cadherin, and cell junctions, affecting biological processes such as tissue development, cell-to-cell adhesion, cell communication, and the immune system. These data are supported by histological observations showing epithelial alterations, subepithelial fibrosis, and collagen deposits in the lungs. These data imply that continuous challenge by the environment and consequent OGG1-BER-driven signaling trigger gene expression consistent with airway remodeling.
KW - 8-Oxoguanine
KW - Airway remodeling
KW - OGG1-BER
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U2 - 10.1016/j.freeradbiomed.2015.07.007
DO - 10.1016/j.freeradbiomed.2015.07.007
M3 - Article
C2 - 26187872
AN - SCOPUS:84941915969
SN - 0891-5849
VL - 89
SP - 20
EP - 33
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
ER -