Abstract
Genetic engineering in mice is one of the most important tools in biomedical research for analysis of gene function. However, there are limitations of murine genetic engineering when it is used for modeling human diseases, due to great differences in body size, lifespan, and species-specific cellular and molecular processes. The nonhuman primate (NHP) exhibits close similarities to human physiology, and NHP experimental models are widely used to assess the safety and efficacy of new drugs prior to human clinical trials. Hence, genetic engineering in NHPs should theoretically be a powerful tool in modeling human diseases and developing novel therapies. The recent development of genome editing technologies such as clustered regularly interspaced short palindromic repeats/CRISPRassociated protein 9 (CRISPR/Cas9) will make genetic engineering in NHPs more efficient and predictable. In this chapter, we summarize conventional genetic engineering technologies as well as CRISPR/Cas9 techniques used in NHPs, and discuss the potential applications of NHP genetic engineering in biomedical research.
| Original language | English (US) |
|---|---|
| Title of host publication | Nonhuman Primate Models in Preclinical Research |
| Subtitle of host publication | Volume 1: Basics and Regulatory Principles |
| Publisher | Nova Science Publishers, Inc. |
| Pages | 187-193 |
| Number of pages | 7 |
| Volume | 1 |
| ISBN (Electronic) | 9781536199222 |
| State | Published - Jan 1 2021 |
Keywords
- CRISPR/Cas9
- Genetic engineering
- Knock out
- Nonhuman primate
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)