Gold Nanoparticles Radio-Sensitize and Reduce Cell Survival in Lewis Lung Carcinoma

Arvind Pandey; Veronica Vighetto; Nicola Di Marzio; Francesca Ferraro; Matteo Hirsch; Nicola Ferrante; Sankar Mitra; Alessandro Grattoni; Carly S. Filgueira

doi:10.3390/nano10091717

Gold Nanoparticles Radio-Sensitize and Reduce Cell Survival in Lewis Lung Carcinoma

Arvind Pandey, Veronica Vighetto, Nicola Di Marzio, Francesca Ferraro, Matteo Hirsch, Nicola Ferrante, Sankar Mitra, Alessandro Grattoni, Carly S. Filgueira

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

It has been suggested that particle size plays an important role in determining the genotoxicity of gold nanoparticles (GNPs). The purpose of this study was to compare the potential radio-sensitization effects of two different sized GNPs (3.9 and 37.4 nm) fabricated and examined in vitro in Lewis lung carcinoma (LLC) as a model of non-small cell lung cancer through use of comet and clonogenic assays. After treatment with 2Gy X-ray irradiation, both particle sizes demonstrated increased DNA damage when compared to treatment with particles only and radiation alone. This radio-sensitization was further translated into a reduction in cell survival demonstrated by clonogenicity. This work indicates that GNPs of both sizes induce DNA damage in LLC cells at the tested concentrations, whereas the 37.4 nm particle size treatment group demonstrated greater significance in vitro. The presented data aids in the evaluation of the radiobiological response of Lewis lung carcinoma cells treated with gold nanoparticles.

Original language	English (US)
Article number	1717
Journal	Nanomaterials
Volume	10
Issue number	9
DOIs	https://doi.org/10.3390/nano10091717 https://doi.org/10.3390/nano10091717
State	Published - Sep 2020

Keywords

Clonogenic assay
Comet assay
Gold nanoparticles
Lewis lung carcinoma
Radio-sensitization

ASJC Scopus subject areas

Chemical Engineering(all)
Materials Science(all)

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@article{3643a609803940619db82416c81fd731,

title = "Gold Nanoparticles Radio-Sensitize and Reduce Cell Survival in Lewis Lung Carcinoma",

abstract = "It has been suggested that particle size plays an important role in determining the genotoxicity of gold nanoparticles (GNPs). The purpose of this study was to compare the potential radio-sensitization effects of two different sized GNPs (3.9 and 37.4 nm) fabricated and examined in vitro in Lewis lung carcinoma (LLC) as a model of non-small cell lung cancer through use of comet and clonogenic assays. After treatment with 2Gy X-ray irradiation, both particle sizes demonstrated increased DNA damage when compared to treatment with particles only and radiation alone. This radio-sensitization was further translated into a reduction in cell survival demonstrated by clonogenicity. This work indicates that GNPs of both sizes induce DNA damage in LLC cells at the tested concentrations, whereas the 37.4 nm particle size treatment group demonstrated greater significance in vitro. The presented data aids in the evaluation of the radiobiological response of Lewis lung carcinoma cells treated with gold nanoparticles.",

keywords = "Clonogenic assay, Comet assay, Gold nanoparticles, Lewis lung carcinoma, Radio-sensitization",

author = "Arvind Pandey and Veronica Vighetto and Marzio, {Nicola Di} and Francesca Ferraro and Matteo Hirsch and Nicola Ferrante and Sankar Mitra and Alessandro Grattoni and Filgueira, {Carly S.}",

note = "Funding Information: This research was funded by the Simmons foundation (A.G.), Golfers Against Cancer (A.G. and C.S.F.), and funds from Houston Methodist Research Institute (S.M., A.G. and C.S.F.). Acknowledgments: The authors would like to thank James Gu, Huie Wang, Wei Jiang, and Weihua Gao for help with AFM, SEM, and TEM imaging, and Corrine Ying Xuan Chua, E. Brian Butler, Danilo Demarchi, and Naomi Halas. Funding Information: Funding: This research was funded by the Simmons foundation (A.G.), Golfers Against Cancer (A.G. and C.S.F.), and funds from Houston Methodist Research Institute (S.M., A.G. and C.S.F.). Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = sep,

doi = "10.3390/nano10091717",

language = "English (US)",

volume = "10",

journal = "Nanomaterials",

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AU - Pandey, Arvind

AU - Vighetto, Veronica

AU - Marzio, Nicola Di

AU - Ferraro, Francesca

AU - Hirsch, Matteo

AU - Ferrante, Nicola

AU - Mitra, Sankar

AU - Grattoni, Alessandro

AU - Filgueira, Carly S.

N1 - Funding Information: This research was funded by the Simmons foundation (A.G.), Golfers Against Cancer (A.G. and C.S.F.), and funds from Houston Methodist Research Institute (S.M., A.G. and C.S.F.). Acknowledgments: The authors would like to thank James Gu, Huie Wang, Wei Jiang, and Weihua Gao for help with AFM, SEM, and TEM imaging, and Corrine Ying Xuan Chua, E. Brian Butler, Danilo Demarchi, and Naomi Halas. Funding Information: Funding: This research was funded by the Simmons foundation (A.G.), Golfers Against Cancer (A.G. and C.S.F.), and funds from Houston Methodist Research Institute (S.M., A.G. and C.S.F.). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/9

Y1 - 2020/9

N2 - It has been suggested that particle size plays an important role in determining the genotoxicity of gold nanoparticles (GNPs). The purpose of this study was to compare the potential radio-sensitization effects of two different sized GNPs (3.9 and 37.4 nm) fabricated and examined in vitro in Lewis lung carcinoma (LLC) as a model of non-small cell lung cancer through use of comet and clonogenic assays. After treatment with 2Gy X-ray irradiation, both particle sizes demonstrated increased DNA damage when compared to treatment with particles only and radiation alone. This radio-sensitization was further translated into a reduction in cell survival demonstrated by clonogenicity. This work indicates that GNPs of both sizes induce DNA damage in LLC cells at the tested concentrations, whereas the 37.4 nm particle size treatment group demonstrated greater significance in vitro. The presented data aids in the evaluation of the radiobiological response of Lewis lung carcinoma cells treated with gold nanoparticles.

AB - It has been suggested that particle size plays an important role in determining the genotoxicity of gold nanoparticles (GNPs). The purpose of this study was to compare the potential radio-sensitization effects of two different sized GNPs (3.9 and 37.4 nm) fabricated and examined in vitro in Lewis lung carcinoma (LLC) as a model of non-small cell lung cancer through use of comet and clonogenic assays. After treatment with 2Gy X-ray irradiation, both particle sizes demonstrated increased DNA damage when compared to treatment with particles only and radiation alone. This radio-sensitization was further translated into a reduction in cell survival demonstrated by clonogenicity. This work indicates that GNPs of both sizes induce DNA damage in LLC cells at the tested concentrations, whereas the 37.4 nm particle size treatment group demonstrated greater significance in vitro. The presented data aids in the evaluation of the radiobiological response of Lewis lung carcinoma cells treated with gold nanoparticles.

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Gold Nanoparticles Radio-Sensitize and Reduce Cell Survival in Lewis Lung Carcinoma

Abstract

Keywords

ASJC Scopus subject areas

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