Abstract
Cancer genome sequencing studies have identified driver genes for a variety of different cancers and helped to understand the genetic landscape of human cancer. It is still challenging, however, to identify cancer driver genes with confidence simply from genetic data alone. In vivo forward genetic screens using Sleeping Beauty (SB) transposon mutagenesis provides another powerful genetic tool for identifying candidate cancer driver genes in wild-type and sensitized mouse tumors. By comparing cancer driver genes identified in human and mouse tumors, cancer driver genes can be identified with additional confidence based upon comparative oncogenomics. This review describes how SB mutagenesis works in mice and focuses on studies that have identified cancer driver genes in the mouse gastrointestinal tract.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2089-2096 |
| Number of pages | 8 |
| Journal | Cancer Science |
| Volume | 112 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2021 |
Keywords
- Sleeping Beauty transposon mutagenesis
- cancer driver genes
- colorectal cancer
- mouse model
- screening
- Genes, Neoplasm/genetics
- Genetic Predisposition to Disease
- Genetic Testing
- Humans
- DNA Transposable Elements/genetics
- Neoplasms/genetics
- Disease Progression
- Gastrointestinal Neoplasms/genetics
- Animals
- Drug Resistance, Neoplasm/genetics
- Mice
- Mutagenesis, Insertional
ASJC Scopus subject areas
- Oncology
- Cancer Research